Low Dose Sophora flavescens Polysaccharide Promote the Migration and Osteogenic Differentiation of Dental Pulp Stem Cells and Up-Regulate the Expression of Osteogenic Genes

This work aimed to investigate the effect of Sophora flavescens polysaccharide on proliferation and osteogenic differentiation dental pulp stem cells. The cells were isolated from rat crown tissue surface biomarkers identified by flow cytometry. was assessed alizarin red staining alkaline phosphatase staining. treated with different doses for time points cell viability counting kit 8. Cell migration detected Transwell wound healing assay. expression collagen I, osteocalcin runt-related transcription factor 2 beta-catenin measured quantitative real polymerase chain reaction assay Western blotting cluster 90 highly expressed 45 expressions relatively low suppressed treatment dose (0.2 mg/ml) promoted cells, but higher concentration (1 10 Treatment up-regulated biomarkers. Low can promote genes.